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rabbit anti munc13 1  (Synaptic Systems)


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    Structured Review

    Synaptic Systems rabbit anti munc13 1
    a Two-color Ex- d STORM images showing a frontal view of a ~7.5-fold expanded active zone in a hippocampal neuron. The sample was treated with 2 µM phorbol 12-myristate 13-acetate (PMA) for 30 min before fixation to increase the probability of detecting active vesicle fusion sites . RIM (CF568, blue) <t>and</t> <t>Munc13-1</t> (AF647, red) are organized in ring-like structures of different degrees that might represent SV docking sites. b Magnified Ex- d STORM images of individual Munc13-1 and RIM arrangements in active zones show regions of varying sub-structures from different synapses categorized in four different states. I Munc13-1 and RIM unorganized. II Only RIM shows ring-like arrangements. III Munc13-1 and RIM are organized in substructures with a diameter >500 nm. IV Munc13-1 and RIM are both organized in ring-like structures with varying diameters <500 nm. c Comparison of substructures, exemplary shown in ( b ) and Supplementary Fig. of all three conditions (PMA, untreated control, DMSO control). Feret’s diameter of structures determined by the polygon tool (Supplementary Fig. ). In state IV, ring-like structures of RIM exhibit a larger diameter (437 ± 69 nm) than Munc13-1 (404 ± 66 nm; n = 43), corresponding to ~48 nm (RIM) and ~44 nm (Munc13-1) diameters of unexpanded structures. The RIM diameter in state II (RIM = 431 ± 55 nm; n = 22) is identical to state IV. Munc13-1 and RIM diameters in state III are similar, with 653 ± 102 nm (RIM) and 661 ± 96 nm (Munc13-1; n = 26) corresponding to diameters of ~83–84 nm of unexpanded structures. Data were obtained from three independent experiments and neuronal cultures for each condition. Scatter dot graphs show single data points, mean ± s.d. P -values of two-sided paired sample t-test (for Munc13-1 versus RIM in state IV, p = 0.0002; Munc13-1 versus RIM in state III, p = 0.75) and two-sided unpaired t-test (for RIM state II versus state IV, p = 0.70) shown as p < 0.001 (***) and p > 0.5 (n.s., non-significant). d Munc13-1 and RIM diameters of ring-like structures determined in state IV (from c ) under different experimental conditions (PMA treated, untreated control and DMSO treated control) show no significant (n.s.) differences (one-way ANOVA with post-hoc Tukey test). Scatter dot graphs show single data points, mean ± s.d. e Overlay of localization clusters ( n = 73) for Munc13-1 and RIM signals. Clusters were determined by DBSCAN and selected based on convex hull area and circularity, shifted by their centroid position and re-binned as overlay figure. The spatial distribution indicates the variety of substructures hiding the center hole that clearly appears in selected clusters. The color scale represents localizations per pixel. Scale bars, a 1 µm; b , e 200 nm. Scale bars show ~7.5× expanded dimensions after re-embedding in the neutral hydrogel.
    Rabbit Anti Munc13 1, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 95/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+munc13-1/126+103/pmc13421506-233-6-9
    Average 95 stars, based on 53 article reviews
    rabbit anti munc13 1 - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Resolving endogenous protein organization in cells with nanometer resolution"

    Article Title: Resolving endogenous protein organization in cells with nanometer resolution

    Journal: Nature Communications

    doi: 10.1038/s41467-026-76146-7

    a Two-color Ex- d STORM images showing a frontal view of a ~7.5-fold expanded active zone in a hippocampal neuron. The sample was treated with 2 µM phorbol 12-myristate 13-acetate (PMA) for 30 min before fixation to increase the probability of detecting active vesicle fusion sites . RIM (CF568, blue) and Munc13-1 (AF647, red) are organized in ring-like structures of different degrees that might represent SV docking sites. b Magnified Ex- d STORM images of individual Munc13-1 and RIM arrangements in active zones show regions of varying sub-structures from different synapses categorized in four different states. I Munc13-1 and RIM unorganized. II Only RIM shows ring-like arrangements. III Munc13-1 and RIM are organized in substructures with a diameter >500 nm. IV Munc13-1 and RIM are both organized in ring-like structures with varying diameters <500 nm. c Comparison of substructures, exemplary shown in ( b ) and Supplementary Fig. of all three conditions (PMA, untreated control, DMSO control). Feret’s diameter of structures determined by the polygon tool (Supplementary Fig. ). In state IV, ring-like structures of RIM exhibit a larger diameter (437 ± 69 nm) than Munc13-1 (404 ± 66 nm; n = 43), corresponding to ~48 nm (RIM) and ~44 nm (Munc13-1) diameters of unexpanded structures. The RIM diameter in state II (RIM = 431 ± 55 nm; n = 22) is identical to state IV. Munc13-1 and RIM diameters in state III are similar, with 653 ± 102 nm (RIM) and 661 ± 96 nm (Munc13-1; n = 26) corresponding to diameters of ~83–84 nm of unexpanded structures. Data were obtained from three independent experiments and neuronal cultures for each condition. Scatter dot graphs show single data points, mean ± s.d. P -values of two-sided paired sample t-test (for Munc13-1 versus RIM in state IV, p = 0.0002; Munc13-1 versus RIM in state III, p = 0.75) and two-sided unpaired t-test (for RIM state II versus state IV, p = 0.70) shown as p < 0.001 (***) and p > 0.5 (n.s., non-significant). d Munc13-1 and RIM diameters of ring-like structures determined in state IV (from c ) under different experimental conditions (PMA treated, untreated control and DMSO treated control) show no significant (n.s.) differences (one-way ANOVA with post-hoc Tukey test). Scatter dot graphs show single data points, mean ± s.d. e Overlay of localization clusters ( n = 73) for Munc13-1 and RIM signals. Clusters were determined by DBSCAN and selected based on convex hull area and circularity, shifted by their centroid position and re-binned as overlay figure. The spatial distribution indicates the variety of substructures hiding the center hole that clearly appears in selected clusters. The color scale represents localizations per pixel. Scale bars, a 1 µm; b , e 200 nm. Scale bars show ~7.5× expanded dimensions after re-embedding in the neutral hydrogel.
    Figure Legend Snippet: a Two-color Ex- d STORM images showing a frontal view of a ~7.5-fold expanded active zone in a hippocampal neuron. The sample was treated with 2 µM phorbol 12-myristate 13-acetate (PMA) for 30 min before fixation to increase the probability of detecting active vesicle fusion sites . RIM (CF568, blue) and Munc13-1 (AF647, red) are organized in ring-like structures of different degrees that might represent SV docking sites. b Magnified Ex- d STORM images of individual Munc13-1 and RIM arrangements in active zones show regions of varying sub-structures from different synapses categorized in four different states. I Munc13-1 and RIM unorganized. II Only RIM shows ring-like arrangements. III Munc13-1 and RIM are organized in substructures with a diameter >500 nm. IV Munc13-1 and RIM are both organized in ring-like structures with varying diameters <500 nm. c Comparison of substructures, exemplary shown in ( b ) and Supplementary Fig. of all three conditions (PMA, untreated control, DMSO control). Feret’s diameter of structures determined by the polygon tool (Supplementary Fig. ). In state IV, ring-like structures of RIM exhibit a larger diameter (437 ± 69 nm) than Munc13-1 (404 ± 66 nm; n = 43), corresponding to ~48 nm (RIM) and ~44 nm (Munc13-1) diameters of unexpanded structures. The RIM diameter in state II (RIM = 431 ± 55 nm; n = 22) is identical to state IV. Munc13-1 and RIM diameters in state III are similar, with 653 ± 102 nm (RIM) and 661 ± 96 nm (Munc13-1; n = 26) corresponding to diameters of ~83–84 nm of unexpanded structures. Data were obtained from three independent experiments and neuronal cultures for each condition. Scatter dot graphs show single data points, mean ± s.d. P -values of two-sided paired sample t-test (for Munc13-1 versus RIM in state IV, p = 0.0002; Munc13-1 versus RIM in state III, p = 0.75) and two-sided unpaired t-test (for RIM state II versus state IV, p = 0.70) shown as p < 0.001 (***) and p > 0.5 (n.s., non-significant). d Munc13-1 and RIM diameters of ring-like structures determined in state IV (from c ) under different experimental conditions (PMA treated, untreated control and DMSO treated control) show no significant (n.s.) differences (one-way ANOVA with post-hoc Tukey test). Scatter dot graphs show single data points, mean ± s.d. e Overlay of localization clusters ( n = 73) for Munc13-1 and RIM signals. Clusters were determined by DBSCAN and selected based on convex hull area and circularity, shifted by their centroid position and re-binned as overlay figure. The spatial distribution indicates the variety of substructures hiding the center hole that clearly appears in selected clusters. The color scale represents localizations per pixel. Scale bars, a 1 µm; b , e 200 nm. Scale bars show ~7.5× expanded dimensions after re-embedding in the neutral hydrogel.

    Techniques Used: Comparison, Control

    Related Articles

    Membrane:

    Article Title: Endogenous SNAP-Tagging of Munc13‑1 for Monitoring Synapse Nanoarchitecture
    Article Snippet: Then, the membrane was blocked for 60 min with blocking buffer (5% ( w / v ) low fat milk in Tris buffered Saline (TBS) buffer supplemented with 0.1% Tween20 (TBS-T)), and blotted with the following antibodies diluted in blocking buffer for 2 h at RT with gentle shaking: Rabbit anti Munc13–1 (Synaptic Systems 126 103, diluted 1:1000), Rabbit anti SNAP (New England Biolabs, P9310S, diluted 1:500), Mouse anti synapsin 1 (Synaptic Systems 106 011, diluted 1:1000), Mouse anti Syntaxin 1A/B (Synaptic Systems 110 011, diluted 1:2000), Rabbit anti Rim1 (Synaptic Systems 140 003, diluted 1:1000).

    Blocking Assay:

    Article Title: Endogenous SNAP-Tagging of Munc13‑1 for Monitoring Synapse Nanoarchitecture
    Article Snippet: Then, the membrane was blocked for 60 min with blocking buffer (5% ( w / v ) low fat milk in Tris buffered Saline (TBS) buffer supplemented with 0.1% Tween20 (TBS-T)), and blotted with the following antibodies diluted in blocking buffer for 2 h at RT with gentle shaking: Rabbit anti Munc13–1 (Synaptic Systems 126 103, diluted 1:1000), Rabbit anti SNAP (New England Biolabs, P9310S, diluted 1:500), Mouse anti synapsin 1 (Synaptic Systems 106 011, diluted 1:1000), Mouse anti Syntaxin 1A/B (Synaptic Systems 110 011, diluted 1:2000), Rabbit anti Rim1 (Synaptic Systems 140 003, diluted 1:1000).

    Saline:

    Article Title: Endogenous SNAP-Tagging of Munc13‑1 for Monitoring Synapse Nanoarchitecture
    Article Snippet: Then, the membrane was blocked for 60 min with blocking buffer (5% ( w / v ) low fat milk in Tris buffered Saline (TBS) buffer supplemented with 0.1% Tween20 (TBS-T)), and blotted with the following antibodies diluted in blocking buffer for 2 h at RT with gentle shaking: Rabbit anti Munc13–1 (Synaptic Systems 126 103, diluted 1:1000), Rabbit anti SNAP (New England Biolabs, P9310S, diluted 1:500), Mouse anti synapsin 1 (Synaptic Systems 106 011, diluted 1:1000), Mouse anti Syntaxin 1A/B (Synaptic Systems 110 011, diluted 1:2000), Rabbit anti Rim1 (Synaptic Systems 140 003, diluted 1:1000).

    Gentle:

    Article Title: Endogenous SNAP-Tagging of Munc13‑1 for Monitoring Synapse Nanoarchitecture
    Article Snippet: Then, the membrane was blocked for 60 min with blocking buffer (5% ( w / v ) low fat milk in Tris buffered Saline (TBS) buffer supplemented with 0.1% Tween20 (TBS-T)), and blotted with the following antibodies diluted in blocking buffer for 2 h at RT with gentle shaking: Rabbit anti Munc13–1 (Synaptic Systems 126 103, diluted 1:1000), Rabbit anti SNAP (New England Biolabs, P9310S, diluted 1:500), Mouse anti synapsin 1 (Synaptic Systems 106 011, diluted 1:1000), Mouse anti Syntaxin 1A/B (Synaptic Systems 110 011, diluted 1:2000), Rabbit anti Rim1 (Synaptic Systems 140 003, diluted 1:1000).

    Immunolabeling:

    Article Title: Endogenous SNAP-Tagging of Munc13‑1 for Monitoring Synapse Nanoarchitecture
    Article Snippet: Then, the membrane was blocked for 60 min with blocking buffer (5% ( w / v ) low fat milk in Tris buffered Saline (TBS) buffer supplemented with 0.1% Tween20 (TBS-T)), and blotted with the following antibodies diluted in blocking buffer for 2 h at RT with gentle shaking: Rabbit anti Munc13–1 (Synaptic Systems 126 103, diluted 1:1000), Rabbit anti SNAP (New England Biolabs, P9310S, diluted 1:500), Mouse anti synapsin 1 (Synaptic Systems 106 011, diluted 1:1000), Mouse anti Syntaxin 1A/B (Synaptic Systems 110 011, diluted 1:2000), Rabbit anti Rim1 (Synaptic Systems 140 003, diluted 1:1000).



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    a Two-color Ex- d STORM images showing a frontal view of a ~7.5-fold expanded active zone in a hippocampal neuron. The sample was treated with 2 µM phorbol 12-myristate 13-acetate (PMA) for 30 min before fixation to increase the probability of detecting active vesicle fusion sites . RIM (CF568, blue) <t>and</t> <t>Munc13-1</t> (AF647, red) are organized in ring-like structures of different degrees that might represent SV docking sites. b Magnified Ex- d STORM images of individual Munc13-1 and RIM arrangements in active zones show regions of varying sub-structures from different synapses categorized in four different states. I Munc13-1 and RIM unorganized. II Only RIM shows ring-like arrangements. III Munc13-1 and RIM are organized in substructures with a diameter >500 nm. IV Munc13-1 and RIM are both organized in ring-like structures with varying diameters <500 nm. c Comparison of substructures, exemplary shown in ( b ) and Supplementary Fig. of all three conditions (PMA, untreated control, DMSO control). Feret’s diameter of structures determined by the polygon tool (Supplementary Fig. ). In state IV, ring-like structures of RIM exhibit a larger diameter (437 ± 69 nm) than Munc13-1 (404 ± 66 nm; n = 43), corresponding to ~48 nm (RIM) and ~44 nm (Munc13-1) diameters of unexpanded structures. The RIM diameter in state II (RIM = 431 ± 55 nm; n = 22) is identical to state IV. Munc13-1 and RIM diameters in state III are similar, with 653 ± 102 nm (RIM) and 661 ± 96 nm (Munc13-1; n = 26) corresponding to diameters of ~83–84 nm of unexpanded structures. Data were obtained from three independent experiments and neuronal cultures for each condition. Scatter dot graphs show single data points, mean ± s.d. P -values of two-sided paired sample t-test (for Munc13-1 versus RIM in state IV, p = 0.0002; Munc13-1 versus RIM in state III, p = 0.75) and two-sided unpaired t-test (for RIM state II versus state IV, p = 0.70) shown as p < 0.001 (***) and p > 0.5 (n.s., non-significant). d Munc13-1 and RIM diameters of ring-like structures determined in state IV (from c ) under different experimental conditions (PMA treated, untreated control and DMSO treated control) show no significant (n.s.) differences (one-way ANOVA with post-hoc Tukey test). Scatter dot graphs show single data points, mean ± s.d. e Overlay of localization clusters ( n = 73) for Munc13-1 and RIM signals. Clusters were determined by DBSCAN and selected based on convex hull area and circularity, shifted by their centroid position and re-binned as overlay figure. The spatial distribution indicates the variety of substructures hiding the center hole that clearly appears in selected clusters. The color scale represents localizations per pixel. Scale bars, a 1 µm; b , e 200 nm. Scale bars show ~7.5× expanded dimensions after re-embedding in the neutral hydrogel.
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    a Two-color Ex- d STORM images showing a frontal view of a ~7.5-fold expanded active zone in a hippocampal neuron. The sample was treated with 2 µM phorbol 12-myristate 13-acetate (PMA) for 30 min before fixation to increase the probability of detecting active vesicle fusion sites . RIM (CF568, blue) and Munc13-1 (AF647, red) are organized in ring-like structures of different degrees that might represent SV docking sites. b Magnified Ex- d STORM images of individual Munc13-1 and RIM arrangements in active zones show regions of varying sub-structures from different synapses categorized in four different states. I Munc13-1 and RIM unorganized. II Only RIM shows ring-like arrangements. III Munc13-1 and RIM are organized in substructures with a diameter >500 nm. IV Munc13-1 and RIM are both organized in ring-like structures with varying diameters <500 nm. c Comparison of substructures, exemplary shown in ( b ) and Supplementary Fig. of all three conditions (PMA, untreated control, DMSO control). Feret’s diameter of structures determined by the polygon tool (Supplementary Fig. ). In state IV, ring-like structures of RIM exhibit a larger diameter (437 ± 69 nm) than Munc13-1 (404 ± 66 nm; n = 43), corresponding to ~48 nm (RIM) and ~44 nm (Munc13-1) diameters of unexpanded structures. The RIM diameter in state II (RIM = 431 ± 55 nm; n = 22) is identical to state IV. Munc13-1 and RIM diameters in state III are similar, with 653 ± 102 nm (RIM) and 661 ± 96 nm (Munc13-1; n = 26) corresponding to diameters of ~83–84 nm of unexpanded structures. Data were obtained from three independent experiments and neuronal cultures for each condition. Scatter dot graphs show single data points, mean ± s.d. P -values of two-sided paired sample t-test (for Munc13-1 versus RIM in state IV, p = 0.0002; Munc13-1 versus RIM in state III, p = 0.75) and two-sided unpaired t-test (for RIM state II versus state IV, p = 0.70) shown as p < 0.001 (***) and p > 0.5 (n.s., non-significant). d Munc13-1 and RIM diameters of ring-like structures determined in state IV (from c ) under different experimental conditions (PMA treated, untreated control and DMSO treated control) show no significant (n.s.) differences (one-way ANOVA with post-hoc Tukey test). Scatter dot graphs show single data points, mean ± s.d. e Overlay of localization clusters ( n = 73) for Munc13-1 and RIM signals. Clusters were determined by DBSCAN and selected based on convex hull area and circularity, shifted by their centroid position and re-binned as overlay figure. The spatial distribution indicates the variety of substructures hiding the center hole that clearly appears in selected clusters. The color scale represents localizations per pixel. Scale bars, a 1 µm; b , e 200 nm. Scale bars show ~7.5× expanded dimensions after re-embedding in the neutral hydrogel.

    Journal: Nature Communications

    Article Title: Resolving endogenous protein organization in cells with nanometer resolution

    doi: 10.1038/s41467-026-76146-7

    Figure Lengend Snippet: a Two-color Ex- d STORM images showing a frontal view of a ~7.5-fold expanded active zone in a hippocampal neuron. The sample was treated with 2 µM phorbol 12-myristate 13-acetate (PMA) for 30 min before fixation to increase the probability of detecting active vesicle fusion sites . RIM (CF568, blue) and Munc13-1 (AF647, red) are organized in ring-like structures of different degrees that might represent SV docking sites. b Magnified Ex- d STORM images of individual Munc13-1 and RIM arrangements in active zones show regions of varying sub-structures from different synapses categorized in four different states. I Munc13-1 and RIM unorganized. II Only RIM shows ring-like arrangements. III Munc13-1 and RIM are organized in substructures with a diameter >500 nm. IV Munc13-1 and RIM are both organized in ring-like structures with varying diameters <500 nm. c Comparison of substructures, exemplary shown in ( b ) and Supplementary Fig. of all three conditions (PMA, untreated control, DMSO control). Feret’s diameter of structures determined by the polygon tool (Supplementary Fig. ). In state IV, ring-like structures of RIM exhibit a larger diameter (437 ± 69 nm) than Munc13-1 (404 ± 66 nm; n = 43), corresponding to ~48 nm (RIM) and ~44 nm (Munc13-1) diameters of unexpanded structures. The RIM diameter in state II (RIM = 431 ± 55 nm; n = 22) is identical to state IV. Munc13-1 and RIM diameters in state III are similar, with 653 ± 102 nm (RIM) and 661 ± 96 nm (Munc13-1; n = 26) corresponding to diameters of ~83–84 nm of unexpanded structures. Data were obtained from three independent experiments and neuronal cultures for each condition. Scatter dot graphs show single data points, mean ± s.d. P -values of two-sided paired sample t-test (for Munc13-1 versus RIM in state IV, p = 0.0002; Munc13-1 versus RIM in state III, p = 0.75) and two-sided unpaired t-test (for RIM state II versus state IV, p = 0.70) shown as p < 0.001 (***) and p > 0.5 (n.s., non-significant). d Munc13-1 and RIM diameters of ring-like structures determined in state IV (from c ) under different experimental conditions (PMA treated, untreated control and DMSO treated control) show no significant (n.s.) differences (one-way ANOVA with post-hoc Tukey test). Scatter dot graphs show single data points, mean ± s.d. e Overlay of localization clusters ( n = 73) for Munc13-1 and RIM signals. Clusters were determined by DBSCAN and selected based on convex hull area and circularity, shifted by their centroid position and re-binned as overlay figure. The spatial distribution indicates the variety of substructures hiding the center hole that clearly appears in selected clusters. The color scale represents localizations per pixel. Scale bars, a 1 µm; b , e 200 nm. Scale bars show ~7.5× expanded dimensions after re-embedding in the neutral hydrogel.

    Article Snippet: The working concentrations for neuron antibodies, rabbit anti- Munc13-1 (Synaptic Systems, no. 126103) and guinea pig anti RIM1/2 (Synaptic Systems, no. 140 205) were 15 μg/ml.

    Techniques: Comparison, Control